Escherichia coli STb Enterotoxin Toxicity and Internalization Investigations: A Mini-Review
نویسندگان
چکیده
منابع مشابه
Escherichia coli heat-stable enterotoxin b (STb) in vivo internalization within rat intestinal epithelial cells.
Heat-stable enterotoxin b (STb) is a low molecular weight toxin known to bind sulfatide, its receptor. The fate of STb bound to rat intestinal epithelium cells was followed using an anti-toxin gold labeled assay and transmission electron microscopy. The data suggest that STb toxin and the fusion protein maltose binding protein (MBP)-STb were internalized whereas its mutant I41 E-M42R with reduc...
متن کاملEffects of Escherichia coli heat-stable enterotoxin STb on intestines of mice, rats, rabbits, and piglets.
There are at least two classes of Escherichia coli heat-stable enterotoxins, STa and STb. Unlike STa, which is active in suckling mice and piglets, STb is inactive in suckling mice but active in piglets and older, weaned pigs. This study examined the activity of STb in several animals and its effect on intestinal histology and cyclic GMP levels in intestinal mucosal cells. STb did not cause flu...
متن کاملExpression of the cloned gene for enterotoxin STb of Escherichia coli.
This study involved the construction of hybrid plasmids to produce heat-stable enterotoxin type II of Escherichia coli (STb). The translation of the open reading frame for the STb gene estA was demonstrated in several ways. Studies using in vivo labeling with [35S]cysteine demonstrated a radiolabeled protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the expected molec...
متن کاملCorrection: Escherichia coli STb Enterotoxin Dislodges Claudin-1 from Epithelial Tight Junctions
Fig 6. Effect of Zn -enrichment on claudin-1 displacement rate. (A) NP-40-soluble and (B) NP-40insoluble fractions. Gray: calcium-free medium, black: Zn-enriched medium, white: Zn-enriched medium treated with STb for 6, 12 and 24 h. Lower panel: Immunoblot showing claudin-1 and GAPDH used to evaluate their relative amounts. NP-40 cell extracted proteins were separated on a 12% acrylamide SDSPAG...
متن کاملEscherichia coli STb Enterotoxin Dislodges Claudin-1 from Epithelial Tight Junctions
Enterotoxigenic Escherichia coli produce various heat-labile and heat-stable enterotoxins. STb is a low molecular weight heat-resistant toxin responsible for diarrhea in farm animals, mainly young pigs. A previous study demonstrated that cells having internalized STb toxin induce epithelial barrier dysfunction through changes in tight junction (TJ) proteins. These modifications contribute proba...
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ژورنال
عنوان ژورنال: Research Journal of Microbiology
سال: 2007
ISSN: 1816-4935
DOI: 10.3923/jm.2007.209.215